منابع مشابه
Thinking outside the TATA box.
When I was doing my PhD research, in the middle of the last century, the nature and complexity of the RNA transcripts of the genome was a topic of lively debate, into which my own work became drawn. The complexity of the mRNA population in a given tissue, celltype or developmental stage—considered as transcripts that were polyadenylated and associated with ribosomes—was estimated to represent s...
متن کاملBidirectional binding of the TATA box binding protein to the TATA box.
By selective attachment of a DNA cleavage agent to specific residues in the yeast TATA box binding protein (yTBP), we demonstrate that, in solution, yTBP binds to the TATA boxes of both the adenovirus major late promoter and the yeast CYC1 promoter with only a modest preference in orientation and binds well to several overlapping binding sites. The general factors TFIIA and TFIIB each increase ...
متن کاملComparing the Performance of Several Popular Machine Learning Algorithms on Classifying TATA-box from putative TATA box
A TATA box is a common transcription binding site that occurs in the upstream of a transcription start site of many genes. Identifying a TATA box accurately is important since it has been shown empirically that a transcription start site (TSS) occurs in the downstream of a TATA box after a fixed distance that is only dependent on the species. Unfortunately, many substrings of a DNA sequence fit...
متن کاملSelective binding of the TATA box-binding protein to the TATA box-containing promoter: analysis of structural and energetic factors.
We report the results of an energy-based exploration of the components of selective recognition of the TATA box-binding protein (TBP) to a TATA box sequence that includes 1) the interaction between the hydrophobic Leu, Pro, and Phe residues of TBP with the TA, AT, AA, TT, and CG steps, by ab initio quantum mechanical calculations; and 2) the free energy penalty, calculated from molecular dynami...
متن کاملGal4-VP16 and Gal4-AH increase the orientational and axial specificity of TATA box recognition by TATA box binding protein.
Previous work has shown that binding of the TATA box binding protein (TBP) to the TATA box is a rate-limiting step during pre-initiation complex (PIC) formation. Although the transcription of eukaryotic genes normally proceeds in one direction, studies in solution have shown that TBP lacks the information necessary to orient itself on the TATA box. Instead, yeast TBP binds TATA-containing promo...
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ژورنال
عنوان ژورنال: Nature
سال: 1992
ISSN: 0028-0836,1476-4687
DOI: 10.1038/360016a0